Research Papers:
Matrine alleviates lipopolysaccharideinduced intestinal inflammation and oxidative stress via CCR7 signal
PDF | Full Text | How to cite
Guojun Wu1,*, Wenhong Zhou2,*, Junfeng Zhao3, Xiaohua Pan1, Yongjie Sun1, Hao Xu1, Peng Shi1, Chong Geng1, Ling Gao4, Xingsong Tian1
1Department of Breast and Thyroid Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, P. R. China
2Department of Nursing, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, P. R. China
3Traffic Police Department, Jinan Public Security Bureau, Jinan 250021, Shandong, P. R. China
4Scientific Center, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, P. R. China
*These authors contributed equally to this work
Correspondence to:
Ling Gao, email: gaoling8822@sina.com
Xingsong Tian, email: xingsong_tianxs@sina.cn
Keywords: matrine, inflammation, oxidative stress, CCR7, LPS
Received: December 10, 2016 Accepted: December 27, 2016 Published: January 11, 2017
ABSTRACT
The aim of this study was to investigate the protective effects of matrine on lipopolysaccharide (LPS)-induced inflammation and oxidative stress in vivo and in vitro. The results showed that matrine improved intestinal inflammatory status and oxidative balance and enhanced chemokine receptor 7 (CCR7) expression. In LPS-challenged mice and Caco-2 cells, matrine alleviated LPS-induced inflammation and oxidative stress via downregulating pro-inflammatory cytokines (IL-1β and IL-17) and malondialdehyde (MDA) production. CCR7-siRNA transfection blocked the protective effects of matrine on LPS-induced inflammation and oxidative stress and exacerbated LPS caused injury. In conclusion, matrine alleviates LPS-induced intestinal inflammation and oxidative stress in mice and Caco-2 cells, which may be associated with CCR7 signal.